T cell subsets CD3/CD4/CD8, counts

This assay provides absolute cell counts and percentages for CD4, CD8 and CD3 T cell subsets.

References

Synonyms

ACD3, CD4, CD4 count, CD8, TAP, Transplant Antibody Panel

Components

Code Name
CD4P % CD4
CD4A Abs CD4 Lymphocyte Cnt
CD8P % CD8
CD8A Abs CD8 Lymphocyte Cnt
CD4CD8 CD4/CD8 Ratio
CD3P % CD3
CD3A Abs CD3 Lymphocyte Cnt
NFDA4 Test Comment

Interpretation

Method

This is a single platform lymphocyte subset assay and has been approved by the U.S. Food and Drug Administration.

Reference Range

See individual components

Ref. Range Notes

%CD4 [33-61]
Abs CD4 [0.730-2.250] Thou/ul
%CD8 [14-35]
Abs CD8 [0.250-1.240] Thou/ul
CD4/CD8 [1.00-3.78]
%CD3 [57-87]
Abs CD3 [1.040-3.400] Thou/ul

Interferences and Limitations

The stability limit for this FDA approved assay is 24 hours

Guidelines

Ordering & Collection

Specimen Type

Collection

3 or 4 mL LAVENDER TOP tube.
Unacceptable: Any gel tube.

Handling Instructions

Specimens must be tested within 24 hours of collection for this FDA assay. For samples received > 24 hours after collection, test will be canceled and a re-draw will be requested. Specimens must be transported at room temperature. Refrigerated or frozen specimens will be canceled and a re-draw will be requested.

Quantity

requested: 3-4 ml
minimum: 1 ml

Processing

Stability: 24 hours @Room Temperature

SPS: Sample can be shared with CBC. Do not add on test if sample will be received in the Hematopathology lab outside the 24-hour stability limit. If sample is approaching 24 hour stability limit, contact hematopathology lab (206-606-7060) to notify them that a specimen is coming and send immediately.

Outside Laboratories: Specimens must be tested within 24 hours of collection. For samples received > 24 hours after collection, test will be canceled and a re-draw will be requested. Contact Lab Med Call Center 206-685-6066 to discuss transportation options. Specimens can be sent directly to the hematopathology lab as well (include contact info).

Performance

LIS Dept Code

Performing Location(s)

UW Hematopathology Laboratory, G7-800
Fred Hutchinson Cancer Center
825 Eastlake Avenue E.
Seattle, WA 98109

Hematopathology hours:
5:30 am - 6:30 pm Monday through Saturday
7:30 am - 4:00 pm Sunday, and with reduced staffing on major holidays